HIGH THROUGHPUT QUANTIFICATION OF
CEFUROXIME SODIUM IN CATTLE PLASMA BY
LC/MS/MS.
CORINA-CIRESICA MONDEA1, LAURIAN VLASE2*, ÁRPAD
GYÉRESI3, MARIUS BOJITA4
1The Pharmacy of the Clinical County Hospital, 2-4, C.Coposu Blvd.,
Sibiu, Romania
2,* Iuliu Haţieganu” University of Medicine and Pharmacy, Faculty of
Pharmacy, Department of Pharmaceutical Technology and
Biopharmaceutics, 12 Ion Creanga St., Cluj Napoca, Romania
3University of Medicine and Pharmacy, Faculty of Pharmacy,
Department of Pharmaceutical Chemistry, 38 Gh. Marinescu St., Targu
Mures, Romania
4“Iuliu Haţieganu” University of Medicine and Pharmacy, Faculty of
Pharmacy, Department of Drugs Analysis, 6 Louis Pasteur St., Cluj-
Napoca, Romania
*corresponding author: vlaselaur@yahoo.com
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Abstract:

A simple, rapid and sensitive LC/MS/MS method for quantification of cefuroxime sodium in cattle plasma has been developed and validated. The analyte was extracted from plasma samples using protein precipitation with a mixture of perchloric acid 12% in acetonitrile. Separation was achieved using a Zorbax SB-C18 column using a mobile phase containing methanol: ammonium acetate 1mM in water (14:86 v/v). The detection was realized in MS-MRM mode using an Ion Trap mass spectrometer with electrospray negative ionization. The linearity domain was established between 63 to 6120ng/ml. Accuracy and precision were less than -4.9% and 9.3% for intra-day assays and -6.9% and 9.3% for inter-day assays, respectively. The recovery ranged between 95 and 105%. The method is very simple and rapid and was used for analysis of cefuroxime sodium in cattle plasma for pharmacokinetics and drug residue analysis.

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