MOLECULAR BIOLOGICAL TECHNIQUES USED
FOR THE IDENTIFICATION OF CANDIDA SPP..
MANUELA-ANDA RADU-POPESCU*1, SILVIA DUMITRIU2,
SIMONA ENACHE-SOARE3, CARMEN BALOTESCU3, DIANA
PELINESCU3, AURELIAN UDRISTOIU4, TATIANA VASSUDIMOV3
1 University of Medicine and Pharmacy “Carol Davila”, Faculty of
Pharmacy, Department of Microbiology, Bucharest, Romania
2 University of Medicine and Pharmacy “Carol Davila”, Faculty of
Dental Medicine, Department of Microbiology, Bucharest, Romania
3Microbiology-Immunology Department, Faculty of Biology, University
of Bucharest, MICROGEN- Center for Research in Microbiology,
Genetics and Biotechnology, University of Bucharest, Faculty of Biology,
Bucharest, Romania
4 Targu Jiu Hospital, Tg. Jiu, Romania
*corresponding author: andamrp@gmail.com
Download Full Article

Abstract:

Early identification of Candida isolates is necessary for an effective antifungal therapy. Conventional identification methods are often difficult and time-consuming. Molecular biological techniques are representing an useful alternative approach. Yeast clinical isolates were obtained from 50 independent patients admitted to the hospital during the year 2008. In the present study yeast identification was based on gene restriction profiles ARNr 5.8S by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and on random amplification profiles, RAPD (Random Amplification of Polymorphic DNA).

Top