QUANTITATIVE ANALYSIS OF
MALONDIALDEHYDE IN NORMAL HUMAN
PLASMA USING FLUORESCENCE AND THE
STANDARD ADDITION METHOD.
MIRUNA SPRINŢEROIU, DAN BĂLĂLĂU, CLAUDIA MARIA
GUŢU, MIHAELA ILIE*, CONSTANTIN PETRARU
”Carol Davila” University of Medicine and Pharmacy, Faculty of
Pharmacy, Toxicology Department, 6 Traian Vuia Str., Bucharest,
Romania
*corresponding author: m16ilie@yahoo.com
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Abstract:

Oxidative stress has been mentioned among the toxic mechanisms of benzodiazepines at the brain level. Additionally, lipid peroxidation leads to the generation of numerous aldehydes, thus transforming them in oxidative stress markers. Malondialdehyde (MDA) was considered for many years to be a reliable marker to indicate the level of free radicals, and that is why the development of robust methods to quantify MDA in biological matrices was and is still considered a priority for clinical biochemistry. The present paper deals with a simple fluorescent method for the quantification of MDA in human plasma using the adduct of MDA with the thiobarbituric acid and the standard addition method. The method allows the quantification of MDA at concentrations of 50 nM, within the range of normal physiological amount of such products in human plasma, avoiding the interference of the matrix.

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