HPTLC QUALITATIVE AND QUANTITATIVE DETECTION OF
STEROLS IN SPECIES OF THE PORTULACA GENUS FROM ROMANIA
ADRIANA IULIANA ANGHEL1, MIHAELA ILIE2, OCTAVIAN TUDOREL OLARU1*, MIHAELA DINU1, ROBERT VIOREL ANCUCEANU1, VIORICA ISTUDOR3
“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, 6 Traian Vuia Street, 020956, Bucharest,
1Pharmaceutical Botany Department
3Pharmacognosy, Phytochemistry and Phytotherapy Department
*corresponding author: firstname.lastname@example.org
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Sterols are secondary metabolites of plants, known for their inhibitory effects on stomach, lung, breast and ovarian cancer.
The intake of phytosterols can enhance the activity of antioxidant enzymes, thus reducing the oxidative stress. The species of
the Portulaca genus from Romania have not been analysed until now for their sterols content. We hereby report on the
qualitative and quantitative analysis of the sterols from Portulaca oleracea L. and Portulaca grandiflora Hook. in view of
their potential therapeutical use.
Alcoholic extracts were obtained from herbal parts of Portulaca grandiflora and Portulaca oleracea, and the HPTLC
analysis was applied to these extracts, using silica gel 60 F254 plates and chloroform – acetone (80:20) as mobile phase; the
densitometric analysis was performed at 206 nm. The sterol amount contained in each sample was calculated and expressed
as β-sitosterol, which was used as external standard. Based on the comparison of Rf values and of the spectra of the spots
with those of the standard, β-sitosterol was identified in both samples. The HPTLC quantitative analysis of the methanolic
extracts showed as much as 79.9 mg β-sitosterol per 100 g of herbal product from Portulaca grandiflora and 73.1 mg per 100
g of herbal product from Portulaca oleracea (on a dry basis).