EFFECT OF PLANT GROWTH REGULATORS ON THE PRODUCTION
OF FLAVONOIDS BY CELL SUSPENSION CULTURES OF DIGITALIS
LANATA

CRISTINA BOTA1*, CONSTANTIN DELIU2
1Department of Drug Industry and Biotechnology, Faculty of Pharmacy, UMF “Iuliu Hatieganu”, 12 I. Creanga Street,
Cluj-Napoca, Romania
2Institute of Biological Researches, Cluj-Napoca
*corresponding author: laurabota69@yahoo.com
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Abstract:
In this paper we investigated the effect of some phytohormones on the production of flavonoids in cell suspension cultures of
Digitalis lanata. There were used two cell lines and different combinations and concentrations of phytohormones. The
highest flavonoids amounts were obtained after 14 days of treatment. Among the tested combinations, the highest yield of
flavonoids, 7.58 mg/g d.w. (dry weight) was obtained, for line 13C-100, with 0.1 mg/L IAA (β-indoleacetic acid) + 1 mg/L
BA (6-benzylaminopurine). For line 11, the most significant increase in flavonoid amount was achieved with 0.1 mg/L NAA
(α-naphtaleneacetic acid) + 1 mg/L BA.






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