HPLC/MS ANALYSIS OF CAFFEIC AND CHLOROGENIC ACIDS
FROM THREE ROMANIAN VERONICA SPECIES AND THEIR
ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES
ANDREI MOCAN1, LAURIAN VLASE2, ANDREEA LETIȚIA ARSENE3*, DAN VODNAR4,
CRISTINA BISCHIN5, RADU SILAGHI-DUMITRESCU5, GIANINA CRIȘAN1
1Department of Pharmaceutical Botany, “Iuliu Hatieganu” University of Medicine and Pharmacy, 8, V. Babes Street, Cluj-
Napoca, Romania
2Department of Pharmaceutical Technology and Biopharmaceutics, “Iuliu Hatieganu” University of Medicine and
Pharmacy, 8, V. Babes Street, Cluj-Napoca, Romania
3Department of Biochemistry, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6, Traian Vuia
Street, sector 2, Bucharest, Romania
4Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary
Medicine, 3-5, Manăştur Street, Cluj-Napoca, Romania
5Department of Chemistry and Chemical Engineering, “Babeș-Bolyai” University, 11, A. Janos Street, Cluj-Napoca,
Romania
*corresponding author: andreeanitulescu@hotmail.com
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Abstract:
Aerial parts of Veronica species are used in Romanian traditional medicine for the treatment of various conditions like kidney
diseases, cough, and catarrh, and are known for their wound-healing properties. In the present study three relevant Veronica
species (Plantaginaceae), namely V. officinalis L., V. teucrium L. and V. orchidea Crantz from the Romanian spontaneous
flora were investigated considering their content in caffeic and chlorogenic acids, antioxidant and antibacterial properties
against anaerobic bacteria. The identification and quantification of the phenolic compounds performed by LC/MS revealed
the presence of the two phenolic acids in all samples. The antioxidant potential investigated by several assays showed V.
officinalis and V. orchidea as superior sources of natural antioxidants. The antibacterial screening against two anaerobic
bacterial strains was assessed by a micro-dilution technique; the results indicating V. teucrium and V. orchidea extracts being
more active on Peptostreptococcus anaerobius.






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