CONTENT


FARMACIA Nr.1, 2008:

  1. DERIVATIVE SPECTROMETRY IN THE ASSAY OF SIMVASTATIN AND LOVASTATIN.
    IOANA DRĂGHICI, ANGELA NEDELCU, CRINA-MARIA MONCIU, CORINA ARAMĂ
    Abstract


  2. EFFECTS OF TWO ACHILLEA SPECIES TINCTURES ON EXPERIMENTAL ACUTE INFLAMMATION.
    MIHAELA POPOVICI, ALINA ELENA PÂRVU, ILIOARA ONIGA, ANCA TOIU, M. TĂMAŞ, DANIELA BENEDEC
    Abstract


  3. STUDY ON INFLUENCE OF CITRIC ACID ON ALBENDAZOLE COMPLEXATION WITH RANDOM METHYLATED-BETA-CYCLODEXTRIN.
    CRISTINA TRANDAFIRESCU, ÁRPÁD GYÉRESI, MIHÁLY KATA, ZOLTÁN AIGNER, CRISTINA EPURE, ZOLTÁN SZABADAI
    Abstract


  4. THE IN VITRO STUDY OF ANTICANCER ACTIVITY OF HORSE CHESTNUT BUDS, AESCULUS HIPPOCASTANUM L.
    CAMELIA PEEV, CRISTINA DEHELEAN, ADRIANA KAYCSA, CODRUŢA ŞOICA, DIANA ANTAL
    Abstract


  5. CONTRIBUTIONS TO THE PHARMACOGNOSTICAL STUDY ON CITRULLI SEMEN.
    MARIA - LIDIA POPESCU, MIHAELA DINU, IOANA TUDOR
    Abstract


  6. SPECTROPHOTOMETRIC DETERMINATION OF SOME BASIC DRUGS IN BULK AND DOSAGE FORMS USING AZO DYES.
    ALEXANDRA FILARETA NEAGU, IOANA CLEMENTINA CONSTANTINESCU, ANGELA NEDELCU, MARINELA FLOREA
    Abstract


  7. REVEARSED PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
    (RP-HPLC) DETERMINATION OF LISINOPRIL AND ITS DEGRADATION PRODUCTS IN STABILITY AND COMPATIBILITY STUDIES.

    DANIELA CONSTANTINESCU, ELENA CUREA
    Abstract


  8. CONTRIBUTIONS TO THE STUDY OF COMPLEX COMBINATION OF CLEMASTINE WITH SODIUM TETRAPHENYLBORATE. NEW GRAVIMETRIC, TITRIMETRIC AND UV SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF CLEMASTINE. NOTE II.
    IOANA CLEMENTINA CONSTANTINESCU
    Abstract


  9. COMPARATIVE STUDY CONCERNING HERBAL DRUGS AND THEIR ANALYSIS METHODOLOGY IN ROMANIAN PHARMACOPOEIA AND EUROPEAN PHARMACOPOEIA
    NOTE II. MONOGRAPHS ABOUT BARKS (HERBAL DRUGS CORTEX –TYPE).

    LIGIA ELENA DUŢU
    Abstract


  10. THIN-LAYER CHROMATOGRAPHY AS ANALYTICAL METHOD FOR INCLUSION COMPLEXES OF SOME DIURETICS WITH CYCLODEXTRINS.
    CODRUŢA ŞOICA, A. GYÉRESI, CRISTINA DEHELEAN, CAMELIA PEEV, Z. AIGNER, M. KATA
    Abstract


  11. SWELLABLE MATRICES WITH CARBOPOLS FOR BENZYDAMINE·HCl DELIVERY FROM BIOADHESIVE OROMUCOSAL TABLETS.
    IULIANA POPOVICI, LĂCRĂMIOARA OCHIUZ, EMANUELA ANTONETA POPA, G. ŞARAMET, V. DORNEANU, A. ŞPAC, D. LUPULEASA
    Abstract


  12. ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRIC INVESTIGATION OF MELISSA OFFICINALIS OIL.
    CLAUDIA C. TOMA, IOAN B. PANCAN, MARIUS CHIRITA, ALINA D. ZAMFIR
    Abstract


  13. THE INFLUENCE OF THIAZOLIDINEDIONES ON MARKERS OF THE ENDOTHELIAL STATUS AT TYPE II DIABETES MELLITUS PATIENTS.
    DENISA MARGINĂ, NICULINA MITREA, DANIELA GRĂDINARU, MARIA VLĂDICĂ, RUXANDRA DĂNCIULESCU
    Abstract




ABSTRACTS:

1. DERIVATIVE SPECTROMETRY IN THE ASSAY OF SIMVASTATIN AND LOVASTATIN.
IOANA DRĂGHICI, ANGELA NEDELCU, CRINA-MARIA MONCIU, CORINA ARAMĂ


University of Medicine and Pharmacy „Carol Davila”, Department of Analytical Chemistry, 6 Traian Vuia str., Bucharest
*corresponding author: ioanafarm@yahoo.com

- Abstract -

A new second-derivative spectrometric method for the assay of two hipolypemic agents, simvastatin and lovastatin, in pharmaceutical dosage forms was developed. The stability of tablets is obtained by adding antioxidants such as buthylhydroxianisol (BHA) and ascorbic acid, and from these mixtures the statines have been studied and quantified. The method was based on the second derivative spectra of simvastatin and lovastatin in methanolic solution. The wavelength range was between 233.2 and 237.7nm. A linear relationship between the second derivative spectra value and the concentration was found between 1.508·10-6 – 15.08·10-6 g/mL lovastatin and 1.02·10-6-10.2·10-6 g/mL simvastatin. Both methods have been validated. The methods have been successfully applied in the determination of the two hipolypemic agents from synthetic mixtures and from pharmaceutical commercial products.

• derivative spectrometry
• simvastatin
• lovastatin

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2. EFFECTS OF TWO ACHILLEA SPECIES TINCTURES ON EXPERIMENTAL ACUTE INFLAMMATION.
MIHAELA POPOVICI1, ALINA ELENA PÂRVU2, ILIOARA ONIGA3, ANCA TOIU1, M. TĂMAŞ1, DANIELA BENEDEC3

University of Medicine and Pharmacy “Iuliu Haţieganu” Cluj-Napoca
1Department of Pharmaceutical Botany, Faculty of Pharmacy, 12, Ion Creangă Street, 400023, Cluj-Napoca
2Department of Pathophysiology
3Department of Pharmacognosy
*corresponding author: PMP_7827@yahoo.com


- Abstract -

Our study investigated the effects of tincture (1:10) from two species of Achillea (Achillea millefolium and Achillea distans) in acute inflammation induced with oil of turpentine (i.m. 0.6mL/100g b.w.) in male Wistar rats. The results were compared with those from a positive control group with experimental inflammation and a group treated with diclofenac (30mg/100g b.w.). The effects were evaluated by measuring total leukocyte count and differential leukocyte count expressed as a percentage, a test of in vitro phagocytosis and the evaluation of nitric oxide synthesis by measuring the metabolites, nitrites and nitrates. Achillea millefolium reduced significantly the monocytes percentage, the activation of circulating phagocytes and NO synthesis had a slight decrease. Achillea distans tincture reduced significantly the total leukocyte and monocyte percentage and the activation of circulating phagocytes. Achillea distans tincture did not influence the NO synthesis. Conclusion: both tinctures had anti-inflammatory effects on bone marrow acute phase response.

• inflammation
• nitric oxide
• phagocytosis
Achillea millefolium
Achillea distans


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3. STUDY ON INFLUENCE OF CITRIC ACID ON ALBENDAZOLE COMPLEXATION WITH RANDOM METHYLATED-BETA-CYCLODEXTRIN.
CRISTINA TRANDAFIRESCU1*, ÁRPÁD GYÉRESI2, MIHÁLY KATA3, ZOLTÁN AIGNER3, CRISTINA EPURE1, ZOLTÁN SZABADAI4

1Victor Babeş University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 1st Eftimie Murgu Str., Timişoara, România
2University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 38th Gheorghe Marinescu Str., Târgu-Mureş, România
3University of Szeged, Faculty of Pharmacy, Department of Pharmaceutical Technology, 6th Eötvös Str., Szeged, Hungary
4Victor Babeş University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Drug Analysis, 1st Eftimie Murgu Str., Timişoara, România
*corresponding author: chimfarmatm@yahoo.com


- Abstract -

Continuing our previous researches regarding the possibility of improving the aqueous solubility of albendazole, the combined effect of random methyl-β-cyclodextrin and citric acid was studied. Ternary complexes of these components in two molar ratio were prepared using usual laboratory methods and the solubility profiles of the complexes were studied in simulated gastric/intestinal medium. The interaction in solid state between the components of the ternary systems was proved by thermal analysis.

• albendazole
• random methylated-β-cyclodextrin

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4. THE IN VITRO STUDY OF ANTICANCER ACTIVITY OF HORSE CHESTNUT BUDS, AESCULUS HIPPOCASTANUM L.
CAMELIA PEEV*, CRISTINA DEHELEAN, ADRIANA KAYCSA, CODRUŢA ŞOICA, DIANA ANTAL

Universitatea de Medicină şi Farmacie “Victor Babeş” Timişoara, P-ţa Eftimie Murgu nr. 2, cod poştal 300041, Timişoara
*corresponding author: cameliapeev@yahoo.com


- Abstract -

The horse chestnut buds are a new category of vegetal products that are used in the modern phytotherapy, also known as gemotherapy. The present study has highlighted the average content of water, total nitrogen, raw protein, haemolytic saponins of buds, as well as the presence of esculin by thin layer chromatography (TLC). The natural compounds, present in small quantities, develop a synergic anticancer activity. This was proved through the compared biotest of Lepidium sativum and, in vitro, on neuroectodermal cell types from skin carcinoma (A 431) and breast cancer (MCF 7). The results show that bud extract develops an anticancer activity.

• horse chestnut
• saponins
• antitumor
• buds

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5. CONTRIBUTIONS TO THE PHARMACOGNOSTICAL STUDY ON CITRULLI SEMEN.
MARIA - LIDIA POPESCU1*, MIHAELA DINU2, IOANA TUDOR1

University of Medicine and Pharmacy ,,Carol Davila“,
Faculty of Pharmacy, 6 Traian Vuia street, Bucharest
1Pharmacognosy. Phytochemistry. Phytotherapy Department
2Pharmaceutical Botany Department
*corresponding author: lidiapopescu24@yahoo.com



- Abstract -

Citrullus lanatus subsp. vulgaris Schard et Zeys Enum, watermelon (Cucurbitaceae) is an annual plant, intensely cultivated in Romania and has been traditionally used as a diuretic. The purpose of this paper is to report on the pharmacognostical research on seeds of this species. The study was carried out on two cultivars: Timuriu of Canada and Lovrin 532. Microscopically we identified the following specific histologic elements: palisade-like epidermal cells with a developed cuticle; epidermal cells with bands of lignine on walls, sclerenchyma, cellulose fibres and sclereids. The chemical analysis has shown the presence of flavones, polysaccharides, triterpenes, sterols, tannins, monosaccharides and non-alkaloid nitrogen containing compounds. The nature of soil seems to have little influence upon the content of flavones (no statistically significant difference was observed between the two cultivars), but has significant influence upon the content of polysaccharides (the seeds of Timuriu of Canada cultivar which are cultivated on the sandy soil are richer in polysaccharides in comparision with those cultivated on the cernoziom soil; the mean difference observed was statistically significant).

Citrulli semen
• flavonoids
• polysaccharides

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6. SPECTROPHOTOMETRIC DETERMINATION OF SOME BASIC DRUGS IN BULK AND DOSAGE FORMS USING AZO DYES.
ALEXANDRA FILARETA NEAGU, IOANA CLEMENTINA CONSTANTINESCU, ANGELA NEDELCU*, MARINELA FLOREA

Department of Analytical Chemistry, Faculty of Pharmacy,
6th Traian Vuia str, Bucharest 2, 020956
*corresponding author: angelanedelcu@yahoo.com


- Abstract -

Considering basic drugs capacity to generate ion pairs with azoic dyes, we investigated the experimental conditions, in order to develop new spectrophotometric methods for their assay, in bulk and in dosage forms. There were used methyl orange and metanil yellow to obtain ion-pairs with amiodarone, astemizole, clemastine, cinnarizine. The ion pairs are highly soluble in low dissociating solvents, such as CHCl3. Extractive chloroformic solutions have characteristic absorbances at 406-425nm, allowing to quantify drug substances in synthetic mixtures and dosage forms. There were established: concentration range, linearity, precision (RDS % < 2), combination ratio (1:1 for all studied complexes) and distribution rate.

• amiodarone
• methyl orange
• astemizole
• metanil yellow
• clemastine
• spectrometry
• cinnarizine

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7. REVEARSED PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
(RP-HPLC) DETERMINATION OF LISINOPRIL AND ITS DEGRADATION PRODUCTS IN STABILITY AND COMPATIBILITY STUDIES.

DANIELA CONSTANTINESCU1*, ELENA CUREA2

1 S.C. Terapia S.A. Cluj
2 University of de Medicine and Pharmacy „ Iuliu Haţieganu” Cluj
*corresponding author: danaeconst@yahoo.com



- Abstract -

Revearsed phase-high performance liquid chromatography (RP-HPLC) method with UV detection for Lisinopril N-[N-(1S)-1-carboxyl-3-phenyilpropyl]-L-Lisyl-L-proline (Lis) was validated and applied on its stability and compatibility studies under excipients influence (lactose, mannitol, calcium phosphate, magnesium stearate, starch) and packaging (PVC, PVDC).
Experimental samples (binary physical mixtures) Lis.2H2O: Excipient mentained at first molecule dehydration temperature were analysed by revearsed phase high performance liquid chromatography, using Lichrospher 100, RP8.
Quantification of anhidrous Lisinopril after these experiments offers informations about how dehydration temperature influences Lis.2H20 stability in excipients and primary package presence.
Practical conclusions of these study concern raw materials selection (mono or dihydrate), technological and preserving conditions on industrial process of Lisinopril tablets obtaining.

• Lisinopril
• RP-HPLC
• Stability

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8. CONTRIBUTIONS TO THE STUDY OF COMPLEX COMBINATION OF CLEMASTINE WITH SODIUM TETRAPHENYLBORATE. NEW GRAVIMETRIC, TITRIMETRIC AND UV SPECTROPHOTOMETRIC METHODS FOR THE ASSAY OF CLEMASTINE. NOTE II.
IOANA CLEMENTINA CONSTANTINESCU

Department of Analytical Chemistry, Faculty of Pharmacy,
6th, Traian Vuia Street, Bucharest 2, 020956, Romania
*corresponding author:constantinescu_clementina@yahoo.com



- Abstract -

The reaction of clemastine fumarate with sodium tetraphenylborate was investigated with the purpose of elaborating new gravimetric, volumetric and UV spectrophotometric assays for clemastine. The methods we have established are accurate and reproducible.

• clemastine
• sodium tetraphenylborate
• gravimetry
• volumetry

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9. COMPARATIVE STUDY CONCERNING HERBAL DRUGS AND THEIR ANALYSIS METHODOLOGY IN ROMANIAN PHARMACOPOEIA AND EUROPEAN PHARMACOPOEIA
NOTE II. MONOGRAPHS ABOUT BARKS (HERBAL DRUGS CORTEX –TYPE).

LIGIA ELENA DUŢU

University of Medicine and Pharmacy ,,Carol Davila“ Bucharest, Faculty of Pharmacy, Pharmacognosy Phytochemistry Phytotherapy Department, 6 Traian Vuia Street, sect. 2,, Bucharest
*corresponding author: ligia.dutu@yahoo.com



- Abstract -

This paper presents a comparative theoretical study about the herbal drugs – cortex type (bark), which have monographs in the10th Edition of Romanian Pharmacopoeia and in the 5th Edition of European Pharmacopoeia (including supplements 5.1 – 5.7), and also their analysis methodology. This study shows that there is one common monograph (Frangulae cortex), and 6 monographs only in European Pharmacopoeia (Cinchonae cortex, Cinnamomi cortex, Pruni africanae cortex, Quercus cortex, Rhamni purshianae cortex, Salicis cortex). For Frangulae cortex the European Pharmacopoeia specification is more complete and the methods (especially for purity evaluation and active principles assay) are more exact and selective than the ones described in Romanian Pharmacopoeia. For herbal drugs which are listed only in European Pharmacopoeia, the specifications include general and specific tests for identity, purity and quality evaluation.

• European Pharmacopoeia
• Romanian Pharmacopoeia
• Analysis methodology
• Cortex

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10. THIN-LAYER CHROMATOGRAPHY AS ANALYTICAL METHOD FOR INCLUSION COMPLEXES OF SOME DIURETICS WITH CYCLODEXTRINS.
CODRUŢA ŞOICA1, A. GYERESI2, CRISTINA DEHELEAN1, CAMELIA PEEV1, Z. AIGNER3, M. KATA3

1 University of Medicine and Pharmacy “Victor Babeş” Timişoara, P-ţa Eftimie Murgu, 2, Romania
2 University of Medicine and Pharmacy Târgu-Mureş, Gh. Marinescu, 34, Romania
3 Institute of Pharmaceutical Technology, University of Szeged, Eötvös 6, Szeged, Hungary
*corresponding author: codrutasoica@yahoo.com


- Abstract -

Thin-layer chromatography has been successfully used for the analysis of cyclodextrin inclusion complexes. As a result of inclusion complexation, the Rf value for stable complexes is significantly lower than Rf values of the active substances.
In the present study, thin-layer chromatography was used for the analysis of inclusion complexes of some sulphonamidic diuretics with different structures (chlorthalidone, hydrochlorothiazide, furosemide, indapamide and acetazolamide) with natural and semisynthetic cyclodextrin (β-cyclodextrin, hydroxypropyl- β-cyclodextrin, randomly methylated- β-cyclodextrin). The complexes were obtained by different preparation methods. There was a significant diminution of the Rf values of the complexes compared with the active substances which can be interpreted as the formation of real inclusion complexes.

• thin-layer chromatography
• diuretics
• cyclodextrin

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11. SWELLABLE MATRICES WITH CARBOPOLS FOR BENZYDAMINE·HCl DELIVERY FROM BIOADHESIVE OROMUCOSAL TABLETS.
IULIANA POPOVICI1, LĂCRĂMIOARA OCHIUZ1, EMANUELA ANTONETA POPA2, G. ŞARAMET2, V. DORNEANU1, A. ŞPAC1, D. LUPULEASA2

1University of Medicine and Pharmacy „Gr. T. Popa” Iaşi, Faculty of Pharmacy, Department of Pharmaceutical Technology, 16 Universităţii st., 700115, Iaşi
2University of Medicine and Pharmacy „C. Davila” Bucharest, Faculty of Pharmacy, Department of Pharmaceutical Technology, 6 Traian Vuia st., 70138, Bucharest
*corresponding author: popoviciiuliana@yahoo.com


- Abstract -

On this study we present our research on the formulation and the characterization of some oromucosal bioadhesive tablets with benzydamine hydrochloride (Bnz·HCl), a local nonsteroidal antiinflammatory drug with multiple effects as: strong antiinflammatory, rapid analgesic, anesthetic, moderate antimicrobial and muscle relaxant. In order to develop a formulation for oromucosal bioadhesive tablets for the treatment of the inflammatory ulcerative diseases of the oral mucosae, which should exhibit o good adhesion „in vivo”, we selected two acrylic polymers: Carbopol 974 P NF and Noveon AA-A-calcium polycarbophilic.
The first aim of this study consists of the preparation and the determination of the physico-chemical characteristics: aspect, dimensions, the mass uniformity, the disintegration time, the hardness, the friability, the pH value, the content of Bnz·HCl, and „in vitro” release of Bnz·HCl from mucoadhesive tablets with Bnz⋅HCl (3mg/tablet). There was selected a formulation which showed the best values of the hardness, the disintegration time and the release of Bnz·HCl aiming to continue the study concerning: the adhesion time, the “in vitro”absorption capacity, the clinical evaluation, and the “in vivo” mucoadhesion capacity of these tablets.

• oromucosal bioadhesive tablets
• carbopols
• physico-chemical characteristics
• „in vitro ” release

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12. ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRIC INVESTIGATION OF MELISSA OFFICINALIS OIL.
CLAUDIA C. TOMA1, IOAN B. PANCAN2, MARIUS CHIRITA3, ALINA D. ZAMFIR2,3*

1Department of Pharmacognosy & Phytotherapy "Victor Babes" University of Medicine and Pharmacy Timisoara, Faculty of Pharmacy, 2 Eftimie Murgu Street, 300041 Romania;
2Department of Chemical and Biological Sciences, „Aurel Vlaicu” University of Arad, Romania;
3 BioMass Spectrometry Laboratory, National Institute for Research and Development in Electrochemistry and Condensed Matter, Timisoara
*corresponding author: zamfir@uav.ro; claudiatoma2004@yahoo.com


- Abstract -

Different dilutions oil-samples of Melissa officinalis L. were analysed by MS/MS, using an High Capacity Ion Trap (HCT) Ultra Post-translational Modification (PTM) mass spectrometer from Bruker (Bruker Daltonik, Bremen, Germany). Comparing the observed mass-spectra a loy of compounds of the oil were identified geranial, neral, 6-methyl-5-hepten-2-one, citronellal, geranyl-acetate, beta-caryophyllene, and beta-caryophyllene-oxide. The fingerprint of the capillary chromatogramm permitted differentiation of the essential oil of Melissa officinalis L.(Lamiaceae Family).

Melissa officinalis
• mass spectrometry
• electrospray
• citronellol

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13. THE INFLUENCE OF THIAZOLIDINEDIONES ON MARKERS OF THE ENDOTHELIAL STATUS AT TYPE II DIABETES MELLITUS PATIENTS.
DENISA MARGINĂ1*, NICULINA MITREA1, DANIELA GRĂDINARU1, MARIA VLĂDICĂ2, RUXANDRA DĂNCIULESCU2

1Department of Biochemistry, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy Bucharest
2“N Paulescu” National Institute for Diabetes and Metabolic Diseases Bucharest
*corresponding author: denisa.margina@gmail.com


- Abstract -

The present study aimed to determine the influence of thiazolidinediones on some markers of the endothelial status (adiponectin level, NO synthesis, intercellular adhesion molecule 1 level), thus evaluating the capacity of the hypoglycemic drugs to activate endogenous anti-atherosclerotic mechanisms.
We designed a prospective clinical study that included 15 hyperglycemic patients. All patients were diagnosed with type II diabetes mellitus and did not respond properly to classical hypoglycemic drugs (biguanides and sulfonylureas).
For these patients the following data was recorded at baseline: body mass index, fasting plasma glucose, adiponectin level, endothelial NO synthesis and intercellular adhesion molecule (ICAM 1) level. The patients received, for two month, a treatment based on thiazolidinediones compounds. At the end of the trial period the same parameters were recorded.
Our results pointed out that the thiazolidinediones compounds have a great influence on the markers illustrating the endothelial status; they improve the endogenous anti-atherosclerotic mechanisms (adiponectin synthesis, NO level) and decrease the expression of inflammatory markers.

• thiazolidinediones
• diabetes mellitus
• endothelium

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